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Test Code VRERP Vancomycin-Resistant Enterococcus, Molecular Detection, PCR, Varies

Reporting Name

VRE PCR

Useful For

Identifying carriers of vancomycin-resistant enterococci

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by vancomycin-resistant Enterococcus DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Perianal, perirectal, rectal, anal

Supplies: Culturette (BBL Culture Swab) (T092)

Container/Tube: Culture transport swab (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)

Specimen Volume: Swab

Specimen Stability Information: Refrigerated (preferred)/Frozen

 

Acceptable:

Specimen Type: Preserved feces

Supplies: Culture and Sensitivity Stool Transport Vial (T058)

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair, Para-Pak C and S vial)

Specimen Volume: Representative portion of feces

Collection Instructions:

1. Collect fresh feces and submit 1 gram or 5 mL in container with transport medium.

2. Place feces in preservative within 2 hours of collection.

Specimen Stability Information: Ambient (preferred)/ Refrigerated

 

Specimen Type: Unpreserved feces

Supplies:

-Stool Container, Small (Random), 4 oz Random (T288)

-Stool Collection Kit, Random (T635)

Container/Tube: Fecal container

Specimen Volume: Representative portion of feces

Collection Instructions: Collect fresh fecal specimen and submit representative sample in fecal container.

Specimen Stability Information: Refrigerated (preferred)/Frozen


Specimen Minimum Volume

See Specimen Required

Reject Due To

E-swab, calcium alginate or, cotton-tipped swab
Swab sent in gel transport medium, viral or universal transport medium
Dry swab
Formalin or PVA fixative		 Reject

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Varies

Reference Values

Negative

Day(s) Performed

Monday through Sunday

CPT Code Information

87500

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VRERP VRE PCR 62261-3

 

Result ID Test Result Name Result LOINC Value
SRC60 Specimen source 31208-2
84406 VRE PCR 62261-3

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

Report Available

1 to 4 days

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.