C. difficile Toxin PCR
Methodology
NUCLEIC ACID AMPLIFICATION BY GENEXPERT CEPHEID
Performing Laboratory
HRRMC
Specimen Requirements
1 mL or more of liquid or semi-formed stool in a plastic or glass container. An acceptable
specimen takes the shape of the container. Store at 2-8° C for up to 5 days in a
clean container, a sterile container is not required. Specimens collected at home may be
placed in a container that has been cleaned in a household dishwasher.
2. Unacceptable specimens include formed stools, rectal swabs, or specimens submitted too
soon after prior testing.
3. Guidelines for repeat testing are:
a. Repeat testing can be performed within 2 days after of an initially-negative result
b. Repeat testing within 2 weeks of a previous-positive result is not acceptable due to
lingering DNA in the absence of infection. Resolution of diarrhea is the best proof of
cure.
c. Accept no more than two specimens/patient without prior consultation with an
individual who can explain the limited yield provided by additional specimens.
d. Do not accept specimens from inpatients after the third hospital day, without prior
consultation.
Specimen Transport Temperature
See above
Reference Values
Negative
Test Classification and CPT Coding
87493